Recent Progress on Cloning and Application of Rice Blast Resistance Genes

doi:10.3969/j.issn.1006-8082.2014.05.001

Abstract

Abstract: Rice blast is one of the most devastating diseases, causing huge losses every year. Exploitation and utilization of broad-spectrum resistance genes are considered as the most effective ways to control the disease. The progress of rice blast resistance gene cloning, functional marker development, marker-assisted breeding in rice were summarized as to provide theoretical guidance for cloning, studying, using rice blast resistance genes.

Key words: Oryza sativa L, rice blast, resistance gene, functional marker, marker-assisted selection

摘要： 稻瘟病是水稻三大病害之一，每年都给水稻生产造成巨大损失，挖掘并合理利用品种自身抗性是解决稻瘟病危害的最有效途径。本文综述了水稻稻瘟病抗性基因克隆、功能标记开发以及稻瘟病抗性分子标记辅助选择育种研究进展，为进一步克隆、研究、应用稻瘟病抗性基因提供理论指导。

关键词: 水稻, 稻瘟病, 抗性基因, 功能标记, 分子标记辅助选择

CLC Number:

S435.111.4+1

ZHANG Pei-Sheng-1, ZHAO Chun-De-2, YU Ning-2, ZHANG Ying-Xin-23, LIU Qun-恩2*. Recent Progress on Cloning and Application of Rice Blast Resistance Genes[J]. , 2014, 20(5): 1-7.

张佩胜1, 赵春德2, 余宁2, 张迎信2,3, 刘群恩2*. 稻瘟病抗性基因的克隆及应用研究进展[J]. 中国稻米, 2014, 20(5): 1-7.

References

[1]    Khush G S. What it will take to feed 5.0 billion rice consumers in 2030[J]. Plant mol biol, 2005, 59（1）: 1-6.

[2]    Scheuermann K K, Raimondi J V, Marschalek R, et al. Magnaporthe oryzae genetic diversity and its outcomes on the search for durable resistance[J]. Mol Basis Plant Genet Divers, 2012: 331-356.

[3]    Shen M, Lin J Y. The economic impact of rice blast disease in China [J]. Rice Blast Disease. RS Zeigler, SA Leong, and PS Teng, eds. CAB International, Wallingford, UK, 1994: 321-331.

[4]    Notteghem J L. Durable resistance to rice blast disease [M]//Durability of disease resistance. Springer Netherlands, 1993: 125-134.

[5]    Miah G, Rafii M Y, Ismail M R, et al. Blast resistance in rice: a review of conventional breeding to molecular approaches [J]. Mol Biol Rep, 2013, 40（3）: 2369-2388.

[6]    Wang Z X, Yano M, Yamanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine‐rich repeat class of plant disease resistance genes [J]. Plant J, 1999, 19（1）: 55-64.

[7]    Wang Z X, Yamanouchi U, Katayose Y, et al. Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences [J]. Plant Mol Biol, 2001, 47（5）: 653-661.

[8]    Bryan G T, Wu K S, Farrall L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J]. Plant Cell, 2000, 12（11）: 2033-2046

[9]    Liu X Q, Lin F, Wang L, et al. The in silico map-based cloning of Pi36, a rice coiled-coil–nucleotide-binding site–leucine-rich repeat gene that confers race-specific resistance to the blast fungus[J]. Genetics, 2007, 176（4）: 2541-2549.

[10] Chen S, Wang L, Que Z, et al. Genetic and physical mapping of Pi37 （t）, a new gene conferring resistance to rice blast in the famous cultivar St. No. 1[J]. Theor Appl Genet, 2005, 111（8）: 1563-1570.

[11] Lin F, Chen S, Que Z, et al. The blast resistance gene Pi37 encodes a nucleotide binding site–leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1[J]. Genetics, 2007, 177（3）: 1871-1880.

[12] Qu S, Liu G, Zhou B, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site–leucine-rich repeat protein and is a member of a multigene family in rice [J]. Genetics, 2006, 172（3）: 1901-1914.

[13] Zhou B, Qu S, Liu G, et al. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea [J]. Mol Plant Microbe In, 2006, 19（11）: 1216-1228.

[14] Deng Y, Zhu X, Xu J, et al. Map-based cloning and breeding application of a broad-spectrum resistance gene Pigm to rice blast [M]//Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer Netherlands, 2009: 161-171.

[15] Wu J L, Fan Y Y, Li D B, et al. Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates[J]. Theor Appl Genet, 2005, 111（1）: 50-56.

[16] Chen X W, Li S G, Xu J C, et al. Identification of two blast resistance genes in a rice variety, Digu [J]. J Phytopathology, 2004, 152（2）: 77-85.

[17] Chen X, Shang J, Chen D, et al. AB‐lectin receptor kinase gene conferring rice blast resistance [J]. Plant J, 2006, 46（5）: 794-804.

[18] Fukuoka S, Okuno K. QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice[J]. Theor Appl Genet, 2001, 103（2-3）: 185-190.

[19] Fukuoka S, Saka N, Koga H, et al. Loss of function of a proline-containing protein confers durable disease resistance in rice[J]. Science, 2009, 325（5943）: 998-1001.

[20] Zhai C, Lin F, Dong Z Q, et al. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication[J]. New Phytol, 2011, 189: 321-334

[21] Ashikawa I, Hayashi N, Yamane H, et al.Two adjacent Nucleotide-Binding Site-Leucine-Rich Repeat Class genes are required to confer Pikm-Specific rice blast resistance [J]. Genetics, 2008, 180: 2267-2276.

[22] Yuan B, Zhai C, Wang W J, et al. The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes [J]. Theor Appl Genet, 2011, 122: 1017-1028.

[23] Sharma T R, Madhav M S, Singh B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea [J]. Mol Gen Genomics, 2005, 274: 569-578.

[24] Hayashi N, Inoue H, Kato T, et al. Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication [J]. Plant J, 2010, 64（3）: 498-510.

[25] Okuyama Y, Kanzaki H, Abe A, et al. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes[J]. Plant J, 2011, 66（3）: 467-479.

[26] Shang J, Tao Y, Chen X, et al. Identification of a new rice blast resistance gene, Pid3, by genomewide comparison of paired nucleotide-binding site--leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes[J]. Genetics, 2009, 182（4）: 1303-1311.

[27] Takahashi A, Hayashi N, Miyao A, et al. Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging[J]. BMC Plant Biol, 2010, 10: 175.

[28] Hayashi K, Yoshida H. Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter[J]. Plant J, 2009, 57（3）: 413-425.

[29] Zhu X, Chen S, Yang J, et al. The identification of Pi50（t）, a new member of the rice blast resistance Pi2/Pi9 multigene family[J]. Theor Appl Genet, 2012, 124（7）: 1295-1304.

[30] Lee S K, Song M Y, Seo Y S, et al. Rice Pi5-mediated resistance to magnaporthe oryzae resence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes[J]. Genetics, 2009, 181（4）: 1627-1638.

[31] Hua L, Wu J, Chen C, et al. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast[J]. Theor Appl Genet, 2012, 125（5）: 1047-1055.

[32] Andersen J R, Lübberstedt T. Functional markers in plants[J]. Trends Plant Sci, 2003, 8（11）: 554-560.

[33] 王忠华，贾育林，吴殿星，等. 水稻抗稻瘟病基因 Pi-ta的分子标记辅助选择[J]. 作物学报，2004，30（12）: 1259-1265.

[34] Jia Y, Singh P. Development of dominant rice blast Pi-ta resistance gene markers[J]. Crop Sci, 2003, 43.

[35] Hayashi K, Yasuda N, Fujita Y, et al. Identification of the blast resistance gene Pit in rice cultivars using functional markers [J]. Theor appl genet, 2010, 121（7）: 1357-1367.

[36] Ramkumar G, Srinivasarao K, Mohan K M, et al. Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54（Pik h）[J]. Mol Breeding, 2011, 27（1）: 129-135.

[37] 刘洋，徐培洲，张红宇，等. 水稻抗稻瘟病 Pib 基因的分子标记辅助选择与应用[J]. 中国农业科学，2008，41（1）：9-14.

[38] 高利军，邓国富，高汉亮，等. 水稻抗稻瘟病基因 Pi-d2 以基因标签的建立与应用[J]. 西南农业学报，2010，23（1）：77-82.

[39] Costanzo S, Jia Y. Sequence variation at the rice blast resistance gene Pi-km locus: Implications for the development of allele specific markers [J]. Plant Sci, 2010, 178（6）: 523-530.

[40] Wang H M, Chen J, Shi Y F, et al. Development and validation of CAPS markers for marker-assisted selection of rice blast resistance gene Pi25[J]. Acta Agronomica Sinica, 2012, 38（11）: 1960-1968.

[41] 高利军，高汉亮，颜群，等. 4 个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布 [C]//《杂交水稻》编辑部.第1届中国杂交水稻大会论文集，2010.

[42] 陈志伟，官华忠，吴为人，等. 稻瘟病抗性基因 Pi-1 连锁 SSR 标记的筛选和应用[J]. 福建农林大学学报：自然科学版，2005，34（1）：74-77.

[43] 金素娟，柳武革，朱小源，等. 利用分子标记辅助选择改良温敏核不育系 GD-8S 的稻瘟病抗性[J]. 中国水稻科学，2007，21（6）：599-604.

[44] 殷得所，夏明元，李进波，等. 抗稻瘟病基因 Pi9 的 STS 连锁标记开发及在分子标记辅助育种中的应用[J]. 中国水稻科学，2011，25（1）：25-30.

[45] 文绍山，高必军. 利用分子标记辅助选择将抗稻瘟病基因 Pi-9 （t）渗入水稻恢复系泸恢 17[J]. 分子植物育种，2012，10（1）：42-47.

[46] 曾正明，况浩池，罗俊涛，等. 利用分子标记辅助筛选改良 ‘泸恢 602’稻瘟病抗性的研究[J]. 浙江农业学报，2012，24（1）：66-70.

[47] Fu C，Wu T, Liu W, et al. Genetic improvement of resistance to blast and bacterial blight of the elite maintainer line Rongfeng B in hybrid rice（Oryza sativa L.） by using marker-assisted selection[J]. African J Biotechnol, 2012, 11（67）: 13104-13114.

[48] 陈红旗，陈宗祥，倪深，等. 利用分子标记技术聚合 3 个稻瘟病基因改良金 23B 的稻瘟病抗性[J]. 中国水稻科学，2008，22（1）：23-27.

[49] Hittalmani S, Parco A, Mew T V, et al. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice [J]. Theor Appl Genet, 2000, 100（7）: 1121-1128.

[50] Chen X W, Li S G, Ma Y Q, et al. Marker-assisted selection and pyramiding for three blast resistance genes, Pi-d （t） 1, Pi-b, Pi-ta2, in rice [J]. Chin J Biotechnol, 2004, 20（5）: 708-714.

[51] 李洪亮，李荣田. 稻瘟病抗性基因 Pi1 和 Pi2 的聚合及其育种价值分析[J]. 北方水稻，2010，40（5）：7-12.

[52] 董巍，李信，晏斌，等. 利用分子标记辅助选择改良培矮 64S 的稻瘟病抗性[J]. 分子植物育种，2010，8（5）：853-860.

[53] 陈志伟，官华忠，王宗华，等. 利用基因聚合技术改良一个优质水稻不育系的稻瘟病抗性[J]. 福建农林大学学报：自然科学版，2012，41（5）：449-454.